Murine alveolar macrophages are highly susceptible to replication of Coxiella burnetii phase II In Vitro

TD Fernandes; LD Cunha; JM Ribeiro; LM Massis; DS Lima-Junior; HJ Newton; DS Zamboni

Journal article

Murine alveolar macrophages are highly susceptible to replication of Coxiella burnetii phase II In Vitro

TD Fernandes, LD Cunha, JM Ribeiro, LM Massis, DS Lima-Junior, HJ Newton, DS Zamboni

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 2016

DOI: 10.1128/IAI.00411-16

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Abstract

Coxiella burnetii is a Gram-negative bacterium that causes Q fever in humans. Q fever is an atypical pneumonia transmitted through inhalation of contaminated aerosols. In mammalian lungs, C. burnetii infects and replicates in several cell types, including alveolar macrophages (AMs). The innate immunity and signaling pathways operating during infection are still poorly understood, in part because of the lack of relevant host cell models for infection in vitro. In the study described here, we investigated and characterized the infection of primary murine AMs by C. burnetii phase II in vitro. Our data reveal that AMs show a pronounced M2 polarization and are highly permissive to C. burnetii mul..

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University of Melbourne Researchers

Hayley Newton Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was supported by grants from the Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP; grants 2013/08216-2, 2014/50268-2, and 2014/04684-4), Conselho Nacional do Desenvolvimento Cientifico e Tecnologico (CNPq), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Fundacao de Amparo ao Ensino, Pesquisa e Assistencia do Hospital das Clinicas da FMRP/USP (FAEPA), and the Australian National Health and Medical Research Council (NHMRC; grants 1062383 and 1063646 to H.J.N.). T.D.F. and L.M.M. are supported by a fellowship from CAPES. L.D.C., J.M.R., and D.S.L. are supported by fellowships from FAPESP. H.J.N. is a visiting professor from the Science without Borders Program (CNPq; grant 401577/2014-7). D.S.Z. is a research fellow from CNPq, Brazil.